The carotenoid profile of SH104 and SHG104 was β-carotene, astaxanthin, canthaxanthin, pheonicoxanthin and echinenone, which analyzed by HPLC and LC/APCI–MS. SHG104 were palmitic acid and a trace of other long-chain fatty acids. In addition to DHA, the main fatty acids produced by Schizochytrium sp. SHG104 yielded 10.8 g L −1 of biomass comprising 45.8% total lipids (32.1% DHA) and 4.6 mg L −1 of astaxanthin. Under combined temperature-shift cultivation conditions employing white-light LEDs (light-emitting diodes), Schizochytrium sp. An improved Schizochytrium mutant, named SHG104, was obtained from the original host strain by γ-irradiation-induced mutagenesis. through 18S rRNA analysis and named SH104, simultaneously produces high levels of DHA and carotenoid-based antioxidant pigments. This newly isolated Thraustochytrid, identified as Schizochytrium sp. In the present study, we describe the isolation and characterization of a new Thraustochytrid Schizochytrium sp. Results to date suggest that microalgal Thraustochytrids family strains can be used to produce high-functional omega-3 rich oil (~ 30–70% of dry cell weight) and carotenoid-based antioxidant pigments simultaneously with value-added bioactive potential.
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